Dr Rohan Ranasinghe

Dr Rohan Ranasinghe

Graduate student (2000-2005)

Rohan received his MChem (2000) and PhD (2005) from the University of Southampton, the latter under the supervision of Tom Brown. From 2005-2008, he worked with Dr Peter Roach in Southampton, synthesising oligonucleotides on encoded microparticles for use in multiplexed assays. Since 2008 he has been carrying out post-doctoral research with Professor David Klenerman at the University of Cambridge, with the aim of developing ultrasensitive point-of-care nucleic acid tests for infectious agents, as part of the European Commission-funded TheraEDGE project. His research interests include nucleic acids chemistry and biophysics, molecular diagnostics, single molecule fluorescence spectroscopy and synthesis of modified oligonucleotides.

6 Papers

Linear fluorescent oligonucleotide probes with an acridine quencher generate a signal upon hybridisation

R. T. Ranasinghe, L. J. Brown and T. Brown. Chem. Commun. 1480-1481, 2001.

Fluorescence based strategies for genetic analysis

R. T. Ranasinghe and T. Brown. Chem. Commun. 5487-5502, 2005.

Recognition of CG inversions in DNA triple helices by methylated 3H-pyrrolo[2,3-d] pyrimidin-2(7H)-one nucleoside analogues

R. T. Ranasinghe, D. A. Rusling, V. E. C. Powers, K. R. Fox and T. Brown. Chem. Commun. 2555-2557, 2005.

Four base recognition by triplex-forming oligonucleotides at physiological pH

D. A. Rusling, V. E. C. Powers, R. T. Ranasinghe, Y. Wang, S. D. Osborne, T. Brown and K. R. Fox. Nucleic Acids Res. 33 (9), 3025-3032, 2005.

Naphthalenyl- and anthracenyl-ethynyl dT analogues as base discriminating fluorescent nucleosides and intramolecular energy transfer donors in oligonucleotide probes

Q. Xiao, R. T. Ranasinghe, A. M. P. Tang and T. Brown. Tetrahedron 63 (17), 3483-3490, 2007.

Ultrasensitive fluorescence-based methods for nucleic acid detection: towards amplification-free genetic analysis

R. T. Ranasinghe and T. Brown. Chem. Commun. 47, 3717-3735, 2011.