Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures
4529-4532, 2018.Chem. Commun. 54,
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.