In a paper published today in Nature Communications, we demonstrate a new approach to the chemical synthesis of libraries of sgRNAs for CRISPR applications. Using the method, standard or chemically modified CRISPR sgRNA libraries can be prepared in a single tube by click ligation of an invariant Cas9-binding 79-mer and a library of variable genome-targeting 20-mers. See our blog post for more information: https://chemistrycommunity.nature.com/users/251216-arun-shivalingam/posts/47615-split-and-click-sgrna.
Ysobel Baker wins poster prize at OTS Munich meeting
Ysobel Baker has won a poster prize at the Oligonucleotide Therapeutic Society meeting in Munich (October 16th 2019) for her work on backbone-modified locked nucleic acids for therapeutic applications. Yssy is funded by a BBSRC grant (Follow-on-Funding).