DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand
Biochim. Biophys. Acta Gene Struct. Expression 1447 (2-3), 137-145, 1999.
Abstract
We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold.